ELISA: Enzyme Linked Immunosorbent Assay


Today you will learn about ELISA – Enzyme Linked Immunosorbent Assay. The enzyme-linked immunosorbent assay (ELISA) is a test that uses antibodies and color change to identify a substance. ELISA is a popular format of “wet-lab” type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries. Antigens from the sample are attached to a surface. Then, a further specific antibody is applied over the surface so it can bind to the antigen. This antibody is linked to an enzyme, and, in the final step, a substance containing the enzyme’s substrate is added. The subsequent reaction produces a detectable signal, most commonly a color change in the substrate.

Here and here you can find interactive ELISA tutorials.

Here is another good interactive presentation.

Also a short video about ELISA

And some theory

Big thanks to http://en.wikipedia.org/wiki/ELISA and https://www.sdstate.edu/sdces/fcs/upload/ELISA_Part1_PPT.pdf/.