The biology olympiad is comprised of two parts: the practical test and the theory test. To prepare for the practicals you need to know how to use different chemicals in the lab. Below you will find a summary of the most important agents used in the biology lab.
Enzymes
Phosphatases
DNA polymerases
DNA ligases
RNA Polymerases
Restriction enzymes
Other
Proteases
Electrophoresis
Agarose gel electrophoresis
- Ethidium bromide
- TAE
- DNA ladder
- Silver: 50x TAE
- Sybr Safe
- Kafatos:DNA 1kbplus ladder
- TBE
- Agarose gel loading buffer
- Cresol Red
- SB
- Xylene cyanol
- Bromophenol blue
PAGE
Buffers
- TAE
- TSS
- PBS
- TE
- Silver: 50x TAE
- Kafatos:10x PBS
- TB
- PIPES
- HEPES
- NEB buffers
- Tris
- Kafatos:P1
- Kafatos:P2
- Kafatos:P3
- B-Per II
- TBE
- Destain
- MES
- SSC
- Phosphate buffer
- SDS sample buffer
- TBS
- TBST
- Killing Buffer
- PBST
- MOPS
- CAPS
- Qiagen Buffers
- S30 buffer
- UA Biophysics:Protocols:PBS
- Tris+Tween
- TE+Tween
- SPRI bead binding buffer
Acids and bases
Other common chemicals
- Ethidium bromide
- Acridine Orange
- IPTG
- ONPF
- Raffinose
- ATc
- Arabinose
- EDTA
- Phenol
- 3-Aminotriazole
- DEPC
- DMPC
- Glucose
- Spermine
- ONPG
- ONP
- PMSF
- Isoamyl alcohol
- X-Gal
- Pefabloc
- AEBSF
- DMF
- Cell and tissue lysis hub
- Cresol Red
- EGTA
- Glycerol
- Bromodeoxyuridine (BrdU)
- Ficoll
- Gibson Assembly
- 1 normal acid or base (1N)
- 2-methylbutane (isopentane)
- Glutaraldehyde (Pentanedial)
- DNA dyes
Fixatives
Reporters
Dyes and stains
- 7-AAD
- Acridine Orange
- Annexin-V
- Carboxyfluorescein Diacetate
- DAPI
- Fluorescein diacetate
- Hoechst 33342
- Propidium iodide
- SYBR Gold
- SYBR Green I
- SYBR Green II
- Sybr Safe
- SYTO
Fluorescent proteins
Enzymes
Detergents
Source: https://openwetware.org/wiki/Materials.
